Generate high-quality multiplexed images that propel your spatial biology research forward.
The Cell DIVE Multiplexed Imaging Solution enables you to produce crystal-clear images of whole tissue using 60+ biomarkers, with automatic calibration and correction to enable robust downstream analysis.
Normal tissue adjacent to colon adenocarcinoma: Tissue was iteratively stained with over 30 CST antibodies and imaged using the Cell DIVE Multiplexed Imaging Solution. Seven biomarkers are shown: GZMB (olive green), PanCK (dark green), CD45 (blue), CD8 (yellow), CD79A (red), CD68 (dark purple), CD11B (violet).
CST and Leica Microsystems have partnered to validate over 100 CST® antibodies on the Cell DIVE Imaging System–testing a growing list of conjugated antibodies to figure out the optimal experimental conditions, so you don't have to.
Save valuable time—avoid the hassle and cost of building, optimizing, and troubleshooting your antibody panel. Use best-in-class CST antibodies, validated for immunohistochemistry (IHC), and rigorously tested for sensitivity and specificity, so you can be confident in your results.
Multiple cancer and normal tissue types were iteratively stained with multiple CST antibodies and imaged using the Cell DIVE Multiplexed Imaging Solution. Optimal intensity values for all biomarkers are shown in one image.
Tailor your antibody panel—choose from the growing list of CST antibodies that have been tested and verified to be compatible for use with the Cell DIVE Multiplexed Imaging Solution.
Quickly customize an antibody panel to suit your needs—choose from the expanding list of antibodies that have already been custom-conjugated to common fluorophores and shown to work on the Cell DIVE Multiplexed Imaging System. Simply reach out to your local sales representative and request the product number and fluorophore listed here. These antibodies are also available in carrier-free formulations (without BSA or azide).
| Target | Product # | Product Name | Clone | Validated Fluorophore | Cell DIVE Dilution |
|---|---|---|---|---|---|
| CD3Ɛ | 85061 | CD3ε (D7A6E) XP® Rabbit mAb | D7A6E | Alexa Fluor® 555 | 1:100 |
| CD8A | 85336 | CD8α (D8A8Y) Rabbit mAb | D8A8Y | Alexa Fluor® 750 | 1:100 |
| CD11B | 49420 | CD11b/ITGAM (D6X1N) Rabbit mAb | D6X1N | Alexa Fluor® 647 | 1:100 |
| CD20 | 48750 | CD20 (E7B7T) XP® Rabbit mAb | E7B7T | Alexa Fluor® 647 | 1:100 |
| CD45 | 13917 | CD45 (Intracellular Domain) (D9M8I) XP® Rabbit mAb | D9M8I | Alexa Fluor® 750 | 1:100 |
| CD79A | 13333 | CD79A (D1X5C) XP® Rabbit mAb | D1X5C | Alexa Fluor® 555 | 1:100 |
| CD163 | 93498 | CD163 (D6U1J) Rabbit mAb | D6U1J | Alexa Fluor® 647 | 1:100 |
| CTLA-4 | 53560 | CTLA-4 (E2V1Z) Rabbit mAb | E2V1Z | Alexa Fluor® 647 | 1:100 |
| FOXP3 | 98377 | FoxP3 (D2W8E) Rabbit mAb | D2W8E | Alexa Fluor® 647 | 1:100 |
| GZMB | 46890 | Granzyme B (D6E9W) Rabbit mAb | D6E9W | Alexa Fluor® 555 | 1:100 |
| Kl-67 | 9027 | Ki-67 (D2H10) Rabbit mAb | D2H10 | Alexa Fluor® 647 | 1:100 |
| NAKATPASE | 23565 | Na,K-ATPase α1 (D4Y7E) Rabbit mAb | D4Y7E | Alexa Fluor® 488 | 1:100 |
| CD56 | 99746 | NCAM1 (CD56) (E7X9M) XP® Rabbit mAb | E7X9M | Alexa Fluor® 647 | 1:100 |
| PD-1 | 86163 | PD-1 (D4W2J) XP® Rabbit mAb | D4W2J | Alexa Fluor® 750 | 1:100 |
| PDL-1 | 13684 | PD-L1 (E1L3N®) XP® Rabbit mAb | E1L3N | Alexa Fluor® 647 | 1:100 |
| SMA | 46469 | α-Smooth Muscle Actin (1A4) Mouse mAb (Alexa Fluor® 488 Conjugate) | 1A4 | Alexa Fluor® 488 | 1:100 |
| SOX9 | 82630 | Sox9 (D8G8H) Rabbit mAb | D8G8H | Alexa Fluor® 488 | 1:100 |
| VIMENTIN | 5741 | Vimentin (D21H3) XP® Rabbit mAb | D21H3 | Alexa Fluor® 750 | 1:100 |
| VIMENTIN | 9854 | Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) | D21H3 | Alexa Fluor® 488 | 1:100 |
Request a custom conjugation to help build your panel—select from the growing list of antibodies, validated on the Cell DIVE Imaging System by Leica Microsystems, that you can request be custom conjugated to compatible fluorophores. These antibodies are also available in carrier-free formulations (without BSA or azide). If you would like to order custom conjugation services, please fill out the Custom Antibody Conjugation Inquiries Form.
Expert CST scientists are ready to provide consultation on the best options for building your antibody panels and can assist you with custom antibody conjugations. You can order any of the antibodies listed in the tables above conjugated to any Leica-compatible fluorophore you choose. Don't know which those are? We can help with that too. With our support, you can get the multiplex images and data you need to move your discovery research forward.
Capturing the Spatial Landscape of Tumor and Immune Cell Lineages in the Microenvironment of Human Cancer Tissues
See how the Cell DIVE Multiplexed Imaging Solution facilitated the iterative staining and imaging of 12 full tissue and TMA slides using more than 30 CST biomarker antibodies. The CST antibody panel enabled the identification of clusters containing cells of different immune classes, cell types, and subtypes.
